Intrinsic gene changes determine the successful establishment of stable renal cancer cell lines from tumour tissue
Hailiang Feng1# , Yu Zhang2# , Kan Liu2, Yan Zhu1, Zhenli Yang1, Xu Zhang2* and Yuqin Liu1*
Int J Cancer. 2017 Mar 3
PMID: 28256713 DOI: 10.1002/ijc.30674
Abstract
Human tumor cell lines are important tools in tumor biological studies, especially those with complete data and follow-up. Clear cell renal cell cancer (ccRCC) is not sensitive to radiotherapy and chemotherapy, and patients with distant metastasis can only rely on targeted therapy. Here we reportestablishment of 7 new ccRCC continouscell lines which were cultured more than 20 generations(which we determine as the criteria of sucessful establishement of continous cell lines) among 81 cases of renal cell cancer. Moreover, gene expression and methylation were profiled with microarrays between the established cell lines and those had a finite in vitro life span of less than 10 generations, as well as cells originated from the same culture but of different generations. Genes includingSLC34A2, SEPP1, SULT1C4 and others were differently expressed in established cell lines and finite cells lines. and changes in their expression might be caused by methylation or demethylation. The expression level of SLC34A2 was related not onlyto the life span in vitro culture but also to tumor size. Additionally six of the seven new ccRCC cell lineshad VHL deletion or termination mutations. So in addition to the establishment of seven new ccRCC cell lines with complete clinical data, we conclude that genes such as SLC34A2 and VHL play key roles in the continuous growth and development of ccRCC.