杜文靜

杜文靜課題組

杜文靜，女，博士生導師

2003-2008年，中國科學(xué)技術(shù)大學(xué)生命科學(xué)學(xué)院，博士學(xué)位

2008-2013年，美國賓夕法尼亞大學(xué)醫學(xué)院，博士后

2013-2015年，美國賓夕法尼亞大學(xué)醫學(xué)院，研究助理

2016年1月起，中國醫學(xué)科學(xué)院基礎醫學(xué)研究所，課題組長(cháng)，博士生導師

2016年入選國家級青年人才項目

2019年7月起聘為北京協(xié)和醫學(xué)院準聘副教授

課題組研究方向：細胞代謝調控與代謝物感知。（1）研究不同生理及病理條件下細胞代謝途徑的調控機制；（2）關(guān)鍵代謝物的感知機制與新功能探索。主要利用細胞、生化、分子生物學(xué)手段和小鼠模型來(lái)探索不同生理及病理條件下代謝調控的規律，揭示多種重大代謝疾病中如糖尿病、脂肪肝、癌癥、心血管疾病等代謝紊亂的原因及其致病機制，通過(guò)研究希望能為重大代謝疾病的治療發(fā)現新策略或新靶點(diǎn)。

主要研究成果：在Nature Metabolism (2022, 2021), Nature Communications (2018), EMBO Rep (2022, 20211)，Science Bulletin (2019)，JBC (2017), Nature (2013), Nature Cell Biology (2013, 2011)等期刊發(fā)表學(xué)術(shù)論文20余篇。

課題組成員：

（1）工作人員：李莉 助理研究員；李薇 主管技師

（2）直博生：   寇俊婕（畢業(yè)于哈爾濱醫科大學(xué)） 張振西（畢業(yè)于華中農業(yè)大學(xué)）；

司玙璠（畢業(yè)于中國農業(yè)大學(xué)）    柳子朝（畢業(yè)于四川大學(xué)）

（3）博士：      楊延婷（畢業(yè)于煙臺大學(xué)）           郭唱（畢業(yè)于四川大學(xué)）

（4）碩士：      蘇靚譽(yù)（畢業(yè)于華中科技大學(xué)）    李皓月（畢業(yè)于南京中醫藥大學(xué)）

             程潤寧（畢業(yè)于蘇州大學(xué)）

（5）協(xié)和4+4臨床試點(diǎn)班 ：秋昱翀（畢業(yè)于北京大學(xué)）

課題組招聘：

課題組長(cháng)期招聘碩士/博士研究生，歡迎對本課題組研究方向感興趣的同學(xué)報考。

課題組招聘1-2名助理/副研究員，1-2名博士后。需熟練掌握分子生物學(xué)與生物化學(xué)實(shí)驗技術(shù)，細胞培養，小鼠模型建立等，能夠在課題組長(cháng)的指導下開(kāi)展課題研究與基金申請工作，協(xié)助課題組長(cháng)指導研究生。

聯(lián)系方式：

中國醫學(xué)科學(xué)院基礎醫學(xué)研究所，細胞生物學(xué)系，可勝大樓457、258

北京市東城區東單三條5號，郵編：100005

電話(huà)：010-69156953

Email: wenjingdu@ibms.pumc.edu.cn;  wjdu123@hotmail.com

課題組照片：

代表論文：

1)  Yao Pengbo#, Chen Taiqi#, Jiang Peng, Li Li, Du Wenjing *. Functional skewing of TRIM21-SIRT5 interplay dictates IL-1β production in DSS-induced colitis. EMBO Reports. 2022 Jun 30;e54391. doi: 10.15252/embr.202154391.

2)  Zhang Zhenxi, Du Wenjing*. Malic enzyme 2 as a therapeutic target for cancer: comments on 'Malic enzyme 2 maintains protein stability of mutant p53 through 2-hydroxyglutarate'. J Mol Cell Biol. 2022 Apr 18;mjac024. doi: 10.1093/jmcb/mjac024.

3)  Zhao Mengjia#, Yao Pengbo#, Mao Youxiang#, Wu Jinjun#, Wang Weihua, Geng Chenhui, Cheng Jie, Du Wenjing*, Jiang Peng*. Malic enzyme 2 maintains protein stability of mutant p53 through 2-hydroxyglutarate. Nature Metabolism. 2022 Feb;4(2):225-238

4)  Sun Huishan#, Li Li#, Li Wei#, Yang Fan, Zhang Zhenxi, Liu Zizhao, Du Wenjing*. p53 transcriptionally regulates SQLE to repress cholesterol synthesis and tumor growth. EMBO Reports. 2021 Aug 30:e52537. doi: 10.15252/embr.202152537

5)  Li Wei #; Kou Junjie #; Qin Junyin #; Li Li; Zhang Zhenxi; Pan Ying; Xue Yi *; Du Wenjing*; NADPH levels affect cellular epigenetic state by inhibiting HDAC3–Ncor complex. Nature Metabolism, 2021, 3(1): 75-89.

6)  Yang Fan#; Kou Junjie#; Li Wei; Du Wenjing*; MYC Enhances Cholesterol Biosynthesis and Supports Cell Proliferation Through SQLE, Frontiers in Cell and Developmental Biology, 2021, 9:655889

7)  Zhang Zhenxi, Su Jingyu, Li Li*, Du Wenjing*. Identification of precise therapeutic targets and characteristic prognostic genes based on immune gene characteristics in uveal melanoma. Frontiers in Cell and Developmental Biology, 2021, doi: 10.3389/fcell.2021.666462

8)  Zhao Mengjia; Jiang Peng; Du Wenjing*; MYC retards cancer cell migration through suppressing fibronectin expression, Science Bulletin, 2019, 64(11): 715-717. 

9)  Li, Le#; Li, Lijia#; Li, Wei; Chen, Taiqi; Bin Zou; Zhao, Lina; Wang, Huili; Wang, Xueying; Xu, Lina; Liu, Xiaohui; Wang, Dong; Li, Bo; Mak, Tak W; Du, Wenjing*; Yang, Xiaolu*; Jiang, Peng*; TAp73-induced phosphofructokinase-1 transcription promotes the Warburg effect and enhances cell proliferation., Nature Communications, 2018, 9(1): 4683-4683. 

10)Yao, Pengbo; Sun, Huishan; Xu, Chang; Chen, Taiqi; Zou, Bing; Jiang, Peng*; Du, Wenjing*; Evidence for a direct cross-talk between malic enzyme and the pentose phosphate pathway via structural interactions, Journal of Biological Chemistry, 2017, 292(41): 17113-17120. 

11)Du, Wenjing#; Jiang, Peng#; Mancuso, Anthony; Stonestrom, Aaron; Brewer, Michael D; Minn, Andy J; Mak, Tak W; Wu, Mian*; Yang, Xiaolu*; TAp73 enhances the pentose phosphate pathway and supports cell proliferation., Nat Cell Biol, 2013, 15(8): 991-1000.  Notes: cover story and featured in News & Views in this issue.

12)Jiang, Peng#; Du, Wenjing#; Mancuso, Anthony; Wellen, Kathryn E; Yang, Xiaolu*; Reciprocal regulation of p53 and malic enzymes modulates metabolism and senescence., Nature, 2013, 493(7434): 689-693.  Notes: Highlighted in journal Cell Research.

13)Jiang, Peng#; Du, Wenjing#; Wang, Xingwu; Mancuso, Anthony; Gao, Xiang; Wu, Mian*; Yang, Xiaolu*; p53 regulates biosynthesis through direct inactivation of glucose-6-phosphate dehydrogenase., Nat Cell Biol, 2011, 13(3): 310-316. Notes: cover story and featured in News & Views in this issue. Highlighted in Nature Chemical Biology.

14)Du, Wenjing#; Jiang, Peng#; Li, Nan; Mei, Yide; Wang, Xingwu; Wen, Longping; Yang, Xiaolu*; Wu, Mian*; Suppression of p53 activity by Siva1., Cell Death Differ, 2009, 16(11): 1493-1504.

15)Jiang, Peng#; Du, Wenjing#; Heese, Klaus; Wu, Mian*; The Bad guy cooperates with good cop p53: Bad is transcriptionally up-regulated by p53 and forms a Bad/p53 complex at the mitochondria to induce apoptosis., Mol Cell Biol, 2006, 26(23): 9071-9082. 

16)Li Le; Mao Youxiang; Zhao Lina; Li Lijia; Wu Jinjun; Zhao Mengjia; Du Wenjing; Yu Li; Jiang Peng*; p53 regulation of ammonia metabolism through urea cycle controls polyamine biosynthesis, Nature, 2019, 567(7747): 253-+.  

17)Jiang, Peng*; Du, Wenjing; Wu, Mian*; Regulation of the pentose phosphate pathway in cancer., Protein Cell, 2014, 5(8): 592-602. 

18)Jiang, Peng; Du, Wenjing; Yang, Xiaolu*; A critical role of glucose-6-phosphate dehydrogenase in TAp73-mediated cell proliferation., Cell Cycle, 2013, 12(24): 3720-3726. 

19)Jiang, Peng; Du, Wenjing; Yang, Xiaolu*; p53 and regulation of tumor metabolism., J Carcinog, 2013, 12: 21-21. 

20)Wang, Xingwu; Zha, Meng; Zhao, Xiaocheng; Jiang, Peng; Du, Wenjing; Tam, Andrew Y H; Mei, Yide*; Wu, Mian*; Siva1 inhibits p53 function by acting as an ARF E3 ubiquitin ligase., Nat Commun, 2013, 4: 1551-1551.

21)Li, Nan; Jiang, Peng; Du, Wenjing; Wu, Zhengsheng; Li, Cong; Qiao, Mengran; Yang, Xiaolu*; Wu, Mian*; Siva1 suppresses epithelial-mesenchymal transition and metastasis of tumor cells by inhibiting stathmin and stabilizing microtubules., Proc Natl Acad Sci U S A, 2011, 108(31): 12851-12856. 

22)Wu, Jiawen; Yang, Yinshan; Zhang, Jiahai; Ji, Peng; Du, Wenjing; Jiang, Peng; Xie, Dinghai; Huang, Hongda; Wu, Mian; Zhang, Guangzhao; Wu, Jihui*; Shi, Yunyu*; Domain-swapped dimerization of the second PDZ domain of ZO2 may provide a structural basis for the polymerization of claudins., J Biol Chem, 2007, 282(49): 35988-35999. 

23)Jiang, Peng; Du, Wenjing; Wu, Mian*; p53 and Bad: remote strangers become close friends., Cell Res, 2007, 17(4): 283-285. 

24)Ma, L; Huang, Y; Song, Z; Feng, S; Tian, X; Du, W; Qiu, X; Heese, K; Wu, M*; Livin promotes Smac/DIABLO degradation by ubiquitin-proteasome pathway., Cell Death Differ, 2006, 13(12): 2079-2088. 

25)Mei, Yide; Du, Wenjing; Yang, Yonghui; Wu, Mian*; Puma(*)Mcl-1 interaction is not sufficient to prevent rapid degradation of Mcl-1., Oncogene, 2005, 24(48): 7224-7237.