范恩國

范恩國，男，博士。2008年獲德國馬丁-路德大學(xué)博士學(xué)位。2008年-2010 年，在德國弗萊堡大學(xué)醫學(xué)院進(jìn)行博士后研究; 2010年-2017年歷任弗萊堡大學(xué)醫學(xué)院助理研究員/課題組長(cháng)及研究員(PI)，期間參與或主持多項德國國家(DFG)課題，總經(jīng)費近200萬(wàn)歐元，并與德國、法國、挪威、奧地利、瑞典、日本等國以及國內的多個(gè)課題組開(kāi)展了密切合作。已培養博士1人(Summa Cum Laude畢業(yè))，醫學(xué)博士1人，碩士數人并指導博士后2人；2017年3月起，任中國醫學(xué)科學(xué)院/北京協(xié)和醫學(xué)院“協(xié)和學(xué)者”特聘教授，任職于病原生物學(xué)系。

鑒于革蘭氏陰性菌外膜上的β-桶狀蛋白在細菌的免疫原性、致病性和耐藥性方面的重要作用，以及其位于陰性菌的最外層因而更易為相關(guān)藥物識別并結合的特點(diǎn)，因此外膜桶狀蛋白是非常理想的新型抗生素作用靶點(diǎn)。故本課題組的研究方向為膜蛋白的生成與演化以及蛋白質(zhì)的跨膜轉運機理，具體為革蘭氏陰性菌外膜β-桶狀蛋白的膜整合與演化和病原蛋白跨細菌外膜的轉運機理研究; 以外膜蛋白為靶點(diǎn)的新型抗生素開(kāi)發(fā)(包括天然產(chǎn)物的分離分析及基于此的藥物篩選) ; 基于蛋白質(zhì)跨細菌外膜轉運的細菌細胞表面蛋白展示技術(shù)為基礎的生物工程產(chǎn)品開(kāi)發(fā)（如疫苗開(kāi)發(fā)等）。

擬使用的研究方法包括蛋白質(zhì)跨膜轉運離體重組系統(versatile in vitro reconstitution system for beta-barrel outer membrane proteins)、蛋白質(zhì)定點(diǎn)光交聯(lián)技術(shù)(site-specific photo-cross-linking)、蛋白質(zhì)的離體合成技術(shù)(in vitro protein transcription/translation system)及相關(guān)的分子生物學(xué)、生物化學(xué)、生物工程及分析化學(xué)與化工、天然產(chǎn)物化學(xué)等。

        目前課題組有助研1名，博士研究生1名，碩士研究生2名，擬聘助研(具博士學(xué)位)1名，有意者請與本課題組聯(lián)系。
        2019年招收推免碩士一名。

聯(lián)系方式：
中國醫學(xué)科學(xué)院/北京協(xié)和醫學(xué)院
基礎醫學(xué)研究所病原生物學(xué)系老科研樓544室
北京市東城區東單三條5號

郵編: 100005
電話(huà)：010-69156984
電子郵件：enguo.fan@ibms.pumc.edu.cn

近5年發(fā)表的論文: 

*通訊作者

1，Chuanyi Yao*, Jinliang Chen, Yinghua Lu, Shaokun Tang, Enguo Fan*. Construction of an asynchronous three-zone simulated-moving-bed chromatography and its application for the separation of vanillin and syringaldehyde, Chemical Engineering Journal，2018, 331:644-651.

2，Shanshan Tian, Shuzhen Zheng, Yanpu Han, Zhenchang Guo, Guijin Zhai, Xue Bai, Xi-Wen He, Enguo Fan, YuKui Zhang, and Kai Zhang. Maleic Anhydride Labeling-based Approach for Quantitative Proteomics and Successive Derivatization of Peptides. Analytical Chemistry, 2017, 89(16):8259-8265.

3, Wenjing Bi, Xue Bai, Fan Gao, Congcong Lu, Ye Wang, Guijin Zhai, Shanshan Tian, Enguo Fan. Yukui Zhang, and Kai Zhang, DNA-Templated Aptamer Probe for Identification of Target Proteins. Analytical Chemistry, 2017, 89(7):4071-4076.

4, Chuanyi Yao*, Kaili Yue, Xueping Ling, Keju Jing, Yinghua Lu, Shaokun Tang and Enguo Fan*. Biocatalytic synthesis of vitamin A palmitate using immobilized lipase produced by recombinant Pichia pastoris, Engineering in Life Sciences, 2017, 17: 768-774.

5, Chengchao Ding, Enguo Fan, Shujuan Wang, Liang Guo, Jie Li, Qing Liu. A potential aquaculture vaccine vector: Evaluation of a double-gene attenuated Listeria monocytogenes in zebrafish ( Danio rerio ), Aquaculture, 2017, 479: 311-320.

6, Dongmei Xi, Jizhen Shang, Enguo Fan, Jinmao You, Shusheng Zhang, and Hua Wang, Nanopore-Based Selective Discrimination of MicroRNAs with Single-Nucleotide Difference Using Locked Nucleic Acid-Modified Probes, Analytical Chemistry, 2016, 88(21): 10540-10546.

7, Xue Bai, Congcong Lu, Jin Jin, Shanshan Tian, Zhenchang Guo, Pu Chen, Guijin Zhai, Shuzhen Zheng, Xiwen He, Enguo Fan, Yukui Zhang, and Kai Zhang. Development of DNA-templated Peptide Probe for Photo-affinity Labeling and Enrichment of the 'Readers' of Histone Modification，Angewandte Chemie International Edition, 2016, 55(28): 7993-7997.

8, Haijuan Zeng, Wenbo Guo, Beibei Liang, Jianwu Li, Xuzhao Zhai, Chunmei Song,   Wenjun Zhao*, Enguo Fan*, Qing Liu*, Self-Paired Monoclonal Antibody Lateral Flow Immunoassay Strip for Rapid Detection of Acidovorax avenae subsp. Citrulli, Analytical and Bioanalytical Chemistry, 2016, 408:6071–6078.

9, Enguo Fan, Nandini Chauhan, Gupta Udatha, Jack Leo, Dirk Linke. Type V secretion systems in bacteria, Microbiology Spectrum, 2016, 3(6): VMBF-0009-2015. in Virulence Mechanisms of Bacterial Pathogens, Fifth Edition, ASM press (Book chapter).

10, Enguo Fan, Derrick Norell, Matthias Müller. An In Vitro Assay for Substrate Translocation by FhaC in Liposomes. Methods in Molecular Biology, 2015, 1329:111-125.

11, Luisa Estrada Mallarino, Enguo Fan, Meike Odermatt, Matthias Müller, Jie Liang, MeiShan Lin, Martin Heinzelmann, Fenja Fritsche, Hans-Jürgen Apell, Wolfram Welte. TtOmp85, a β-Barrel Assembly Protein, Functions by Barrel Augmentation. Biochemistry, 2015, 54 (3): 844–852.

12, Yanfang Wu, Xinsheng Wang, Enguo Fan*, Extraction, Structure, and Bioactivities of the Polysaccharides from Fructus. Book chapter in Polysaccharides: Bioactivity and Biotechnology (Edited by Jean-Michel Merillon and Kishan G. Ramawat), Springer Verlag, Germany, 2015, 1585-1607 (Book chapter).

13, Man Wu,   Haolin Li,   Yinghui Jiang,   Chunmei Song,   Huiqin Guo,   Shuyan Wu,   Cheng Liu,  Jianwu Li,  Haijuan Zeng,  Xuzhao Zhai,  Weisheng Zhang,*  Enguo Fan* and  Qing Liu*. Simultaneous quantification of Escherichia coli O157:H7 and Shigella boydii using a visual-antibody-macroarray. Analyst, 2015, 140(19):6595-601.

14, Ye Wang, Yanpu Han, Enguo Fan*, Kai Zhang*. Analytical strategies used to identify the readers of histone modifications: A review. Analytica Chimica Acta, 2015, 891:32-42.

15, Yanpu Han, Congcong Lu, Kai Zhang, Shanshan Tian, Enguo Fan, Langxing Chen, Xi-Wen He and Yukui Zhang. Quantitative characterization of histones post-translational modifications using stable isotope dimethyl-labeling strategy. Analytical Methods, 2015, 7: 3779-3785.

16, Derrick Norell, Alexander Heuck, Thuy-Anh Tran-Thi, Hansj?rg G?tzke, Fran?oise Jacob-Dubuisson, Tim Clausen, Daniel O. Daley, Volkmar Braun, Matthias Müller*, Enguo Fan*, Versatile in vitro system to study translocation and functional integration of bacterial outer membrane proteins. Nature Communications, 2014, 5:5396.

17, Kai Zhang, Liyan Li, Mengxiao Zhu, Guojuan Wang, Jianjun Xie, Yunlong Zhao, Enguo Fan, Liyan Xu, Enmin Li, Comparative analysis of histone H3 and H4 post-translational modifications of esophageal squamous cell carcinoma with different invasive capabilities. Journal of Proteomics, 2014, 2014, 112C:180-189.

18, Qiang Wang, Xin Zhao, Yanrong Ren, Enguo Fan, Haijun Chang, Hongbin Wu, Effects of high pressure treatment and temperature on lipid oxidation and fatty acid composition of yak (Poephagus grunniens) body fat. Meat Science. 2013, 94(4):489-494.

19, Changzhen Wang, Hongmei Zhou, Ruiyun Peng, Lifeng Wang, Zhentao Su, Peng Chen, Shuiming Wang, Shaoxia Wang, Yao Liu, Jianbo Cong, Ke Wu*, Xiangjun Hu*, Enguo Fan*, Electromagnetic Pulse Reduces Free Radical Generation in Rat Liver Mitochondria In Vitro, Free Radical Research 2013, 47(4): 276-82.

20, Kai Zhang*, Shanshan Tian, Enguo Fan*, Protein lysine acetylation analysis: current MS-based proteomic technologies. Analyst. 2013, 138 (6), 1628-1636.

21, Yanfang Wu, Xinsheng Wang, Biao Shen, Lei Kang, Enguo Fan*, Extraction of polysaccharides from plant. Recent Patents on Food, Nutrition & Agriculture, 2013, 5: 57-61.

22, Enguo Fan, Silke Fiedler, Francoise Jacob-Dubuisson, Matthias Müller, Two-partner secretion of gram-negative bacteria: A single β-barrel protein enables transport across the outer membrane, Journal of Biological Chemistry, 2012, 287(4): 2591-2599.

23, Yanfang Wu, Xinsheng Wang, Enguo Fan*, Optimization of ultrasound-assisted extraction of puerarin and total isoflavone from puerariae lobatae radix (Pueraria lobata (Wild.) Ohwi) with response surface methodology, Phytochemical Analysis, 2012, 23: 513–519.